immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from brucella melitensis

نویسندگان

jafar amani applied microbiology research center, baqiyatallah university of medical sciences, tehran, iran

amir ghasemi molecular biology research center, baqiyatallah university of medical sciences, tehran, iran

reza ranjbar molecular biology research center, baqiyatallah university of medical sciences, tehran, iran

mahdi shabani monoclonal antibody research center, avicenna research institute, acecr, tehran, iran

چکیده

objective(s): brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. immunity against brucella melitensis involves antigen-specific cd4+ and cd8+ t-cells activation and humoral immune responses. due to negative aspects of live attenuated vaccines, much attention has been focused on finding brucella-protective antigens to introduce them as potential subunit vaccine candidates. materials and methods: a chimeric gene encoding trigger factor (tf), omp3148-74 and bp2687-111 fragments (tob) from b. melitensis was successfully cloned, expressed in escherichia colibl21-de3 and purified by ni-nta agarose column. antibodies to recombinant tob (rtob) have been investigated in brucella-infected human sera and a pool serum prepared from b. melitensis-vaccinated rabbits. results: our results showed that the immunized rabbit pool serum strongly reacted with rtob. in addition, antibodies against rtob were detectable in 76.5% of sera obtained from infected patients. conclusion: these findings suggest that rtob may provide a potential immunogenic candidate which could be considered in future vaccine studies.

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Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis

Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antig...

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Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis

OBJECTIVES Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4(+) and CD8(+) T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective anti...

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۴، صفحات ۳۵۰-۳۵۵

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